The evaluation and comparison of the elastic modulus of mechanically motivated and pure rabbit mesenchymal stem cell with mature chondrocytes

Authors

  • Shahrokh Shojaei
  • Amir Shirazian
  • Azadeh Jahanbakhsh
  • Hanie Kavand
  • Nooshin Haghighipour

DOI:

https://doi.org/10.22034/JATE.2018.22

Keywords:

Stem cell, chondrocytes, Hydrostatic pressure, Elastic modulus, AFM

Abstract

Background: Different cells are located in different anatomical location of the body and consequently they are exposed to different mechanical forces. Chondrocytes, which form the cartilage tissue, are located in the articular joints like knee and they are daily influenced by intermittent hydrostatic pressure of thousands of times. Mechanical stimulations of the cells have vital contributions on acquisition of their functional characteristics, particularly, elasticity of the cells, which is defined by elastic modulus is one the most imperative mechanical properties. The arrangement and organization of actin fibers in the cytoskeleton can determine the elasticity of the cells, and it has been illustrated that it plays a pivotal role in some important cellular activities such as motility or cell- cell interaction.

Methods and Materials:  In this research, the stem cells and mature chondrocytes were extracted from rabbit adipose and cartilage tissue, respectively.A unique bioreactor was used to apply intermittent hydrostatic pressure (0-1 MPa, 0.5 Hz) to Rabbit Mesenchymal Stem Cells (RMSCs). After applying the forces, the elastic modulus of different groups of cells were assessed and compared by atomic force microscopy.

Results: The results demonstrated that applying the hydrostatic pressure can modify the elastic modulus of stem cells up to 90% and make them more resemble to the mature chondrocytes.

Conclusion: The result of this paper can be important for applications of cartilage tissue engineering.

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Published

2019-12-10

How to Cite

Shojaei, . S. ., Shirazian, A. ., Jahanbakhsh, A. ., Kavand, H. ., & Haghighipour, N. . (2019). The evaluation and comparison of the elastic modulus of mechanically motivated and pure rabbit mesenchymal stem cell with mature chondrocytes. The Journal of Applied Tissue Engineering, 5(1), 37–43. https://doi.org/10.22034/JATE.2018.22

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Original Articles